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KMID : 0848120130380020067
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International Journal of Oral Biology
2013 Volume.38 No. 2 p.67 ~ p.72
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The Molecular Mechanism of Baicalin on RANKL-induced Osteoclastogenesis in RAW264.7 Cells
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Ko Seon-Yle
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Abstract
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This study examined the anti-osteoclastogenic effects ofbaicalin on receptor activator of NF-kB ligand (RANKL)-induced RAW264.7 cells. Baicalin is a flavonoid that isproduced by Scutellaria baicalensis and is known to havemultiple biological properties, including antibacterial, antiinflammatoryand analgesic effects. The effects of baicalinon osteoclasts were examined by measuring 1) cell viability;2) the formation of tartrate-resistant acid phosphatase(TRAP) (+) multinucleated cells; 3) RANK/RANKL signalingpathways and 4) mRNA levels of osteoclast-associatedgenes. Baicalin inhibited the formation of RANKL-stimulatedTRAP (+) multinucleated cells and also suppressedthe RANKL-stimulated activation of p-38, ERK, cSrc andAKT signaling. Baicalin also inhibited the RANKL-stimulateddegradation of IĸB in RAW264.7 cells. In addition,the RANKL-stimulated induction of NFATc1 transcriptionfactors was found to be abrogated by this flavonoid. Baicalinwas further found to decrease the mRNA expression ofosteoclast-associated genes, including carbonic anhydraseII, TRAP and cathepsin K in the RAW264.7 cells. Our datathus demonstrate that baicalin inhibits osteoclastogenesis byinhibiting the RANKL-induced activation of signalingmolecules and transcription factors in osteoclast precursors.
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KEYWORD
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Baicalin, Osteoclastogenesis, MAPK, NFATc1
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